myc-hIFITM3-delta1-21
(Plasmid #104365)

• Purpose: Expresses human IFITM3-delta1-21 with an N-terminal myc tag in mammalian cells.
• Depositing Lab: Jacob Yount
• Publication: Chesarino et al PLoS Pathog. 2015 Aug 11;11(8):e1005095. doi: 10.1371/journal.ppat.1005095. eCollection 2015 Aug.

Backbone

• Vector backbone: pCMV-myc
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 3800
• Total vector size (bp): 4139
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: IFITM3
• Species: H. sapiens (human)
• Insert Size (bp): 339
• Mutation: deleted amino acids 1-21
• Entrez Gene: IFITM3 (a.k.a. 1-8U, DSPA2b, IP15)
• Promoter: CMV
• Tag / Fusion Protein:
  • myc (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Sal (not destroyed)
• 5′ sequencing primer: GATCCGGTACTAGAGGAACTGAAAAAC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry