pEMS2143
(Plasmid #104356)

• Purpose: AAV plasmid with smCBA promoter driving expression of emerald GFP (EmGFP) reporter. Contains WPRE.
• Depositing Lab: Elizabeth Simpson
• Publication: Simpson et al Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.

Backbone

• Vector backbone: AAV2
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: ssAAV-smCBA-EmGFP-WPRE
• Alt name: EmGFP
• Species: Synthetic
• Promoter: smCBA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pCAG-F
• 3′ sequencing primer: WPRE-R

Resource Information

• Supplemental Documents:
  • pEMS2143.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the annotated GenBank file and the additional image file for the specific location of features in this plasmid.

For additional information, please see de Leeuw et al., PMID 27164903