pCSJ95
(Plasmid #104343)

• Purpose: Express yeast Fbp1-3xflag and reference flag-DHFR-ha protein, both are contolled by tetracycline mediated TDH promter, test protein expression and stability.
• Depositing Lab: Alexander Varshavsky
• Publication: Chen et al Science. 2017 Jan 27;355(6323). pii: 355/6323/eaal3655. doi: 10.1126/science.aal3655.

Backbone

• Vector backbone: pJO629
• Backbone size w/o insert (bp): 8017
• Vector type: Yeast Expression
• Selectable markers: HIS3

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FBP1
• Alt name: Fructose-1,6-bisphosphatase
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 1047
• Tag / Fusion Protein:
  • 3xFLAG (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AscI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: M13-forward
• 3′ sequencing primer: M13-reverse

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Replace the Yap5 gene of pJO629 to FBP1 gene.