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Addgene

pTUP1
(Plasmid #104341)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104341 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS316
  • Backbone size w/o insert (bp) 4887
  • Vector type
    Yeast Expression
  • Selectable markers
    URA3

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    TUP1
  • Alt name
    S000000680
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2142
  • Entrez Gene
    TUP1 (a.k.a. YCR084C, AAR1, AER2, AMM1, CRT4, CYC9, FLK1, ROX4, SFL2, UMR7)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Spe I (unknown if destroyed)
  • 3′ cloning site Cla I (unknown if destroyed)
  • 5′ sequencing primer M13-forward
  • 3′ sequencing primer M13-reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTUP1 was a gift from Alexander Varshavsky (Addgene plasmid # 104341 ; http://n2t.net/addgene:104341 ; RRID:Addgene_104341)

  • For your References section:

    Amino acids induce peptide uptake via accelerated degradation of CUP9, the transcriptional repressor of the PTR2 peptide transporter. Xia Z, Turner GC, Hwang CS, Byrd C, Varshavsky A. J Biol Chem. 2008 Oct 24;283(43):28958-68. doi: 10.1074/jbc.M803980200. Epub 2008 Aug 15. 10.1074/jbc.M803980200 PubMed 18708352
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