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Addgene

pSSN6
(Plasmid #104340)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104340 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pRS314
  • Backbone size w/o insert (bp) 4783
  • Vector type
    Yeast Expression
  • Selectable markers
    TRP1

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SSN6
  • Alt name
    CYC8
  • Alt name
    S000000316
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
    2901
  • Entrez Gene
    CYC8 (a.k.a. YBR112C, CRT8, SSN6)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (unknown if destroyed)
  • 3′ cloning site EcoRI (unknown if destroyed)
  • 5′ sequencing primer M13-forward
  • 3′ sequencing primer M13-reverse
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS result found SSN6 with P40Q variant.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSSN6 was a gift from Alexander Varshavsky (Addgene plasmid # 104340 ; http://n2t.net/addgene:104340 ; RRID:Addgene_104340)

  • For your References section:

    Amino acids induce peptide uptake via accelerated degradation of CUP9, the transcriptional repressor of the PTR2 peptide transporter. Xia Z, Turner GC, Hwang CS, Byrd C, Varshavsky A. J Biol Chem. 2008 Oct 24;283(43):28958-68. doi: 10.1074/jbc.M803980200. Epub 2008 Aug 15. 10.1074/jbc.M803980200 PubMed 18708352
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