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Addgene

pCB410 (His-Ub-Ate1-4 1A7B)
(Plasmid #104339)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104339 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pH10UE
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ATE1
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Ate1 (a.k.a. AI225793, AW547406)
  • Tag / Fusion Protein
    • 6xHis-Ub (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer T7
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found plasmid contains Ate1 isoform 2 [NM_001271343.1].

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCB410 (His-Ub-Ate1-4 1A7B) was a gift from Alexander Varshavsky (Addgene plasmid # 104339 ; http://n2t.net/addgene:104339 ; RRID:Addgene_104339)
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