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Addgene

pBL119-2
(Plasmid #104336)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104336 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    ColE1
  • Total vector size (bp) 5531
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB10b
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Thiosulfate response regulator
  • Alt name
    ThsR
  • Species
    Shewanella halifaxensis HAW-EB4
  • Insert Size (bp)
    612
  • GenBank ID
    ABZ77676
  • Promoter PLTetO1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsaI (destroyed during cloning)
  • 3′ cloning site BsaI (destroyed during cloning)
  • 5′ sequencing primer CGTCTAAGAAACCATTATTATCATGACATTAAC
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBL119-2 was a gift from Jeffrey Tabor (Addgene plasmid # 104336 ; http://n2t.net/addgene:104336 ; RRID:Addgene_104336)

  • For your References section:

    Phosphatase activity tunes two-component system sensor detection threshold. Landry BP, Palanki R, Dyulgyarov N, Hartsough LA, Tabor JJ. Nat Commun. 2018 Apr 12;9(1):1433. doi: 10.1038/s41467-018-03929-y. 10.1038/s41467-018-03929-y PubMed 29650958
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