mGarnet2-alpha-tubulin
(Plasmid
#104318)
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Purposecodes for a fusion of fluorescent protein mGarnet2 and alpha-tubulin, visualizes alpha-tubulin
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104318 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepEGFP
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namemGarnet2-alpha-tubulin
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Insert Size (bp)2082
- Promoter CMV
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer CMV-F (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
mGarnet2-alpha-tubulin was a gift from Ulrich Nienhaus (Addgene plasmid # 104318 ; http://n2t.net/addgene:104318 ; RRID:Addgene_104318) -
For your References section:
A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy. Matela G, Gao P, Guigas G, Eckert AF, Nienhaus K, Nienhaus GU. Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h. 10.1039/c6cc09081h PubMed 28044150