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Addgene

alpha-actinin-mGarnet2
(Plasmid #104316)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104316 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/ life technologies
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-actinin-mGarnet2
  • Insert Size (bp)
    3408
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha-actinin-mGarnet2 was a gift from Ulrich Nienhaus (Addgene plasmid # 104316 ; http://n2t.net/addgene:104316 ; RRID:Addgene_104316)

  • For your References section:

    A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy. Matela G, Gao P, Guigas G, Eckert AF, Nienhaus K, Nienhaus GU. Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h. 10.1039/c6cc09081h PubMed 28044150
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