Skip to main content
Addgene

alpha-actinin-mGarnet
(Plasmid #104315)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104315 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/ life technologies
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    alpha-actinin-mGarnet
  • Insert Size (bp)
    3408
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site PvuI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    alpha-actinin-mGarnet was a gift from Ulrich Nienhaus (Addgene plasmid # 104315 ; http://n2t.net/addgene:104315 ; RRID:Addgene_104315)

  • For your References section:

    Monomeric Garnet, a far-red fluorescent protein for live-cell STED imaging. Hense A, Prunsche B, Gao P, Ishitsuka Y, Nienhaus K, Nienhaus GU. Sci Rep. 2015 Dec 9;5:18006. doi: 10.1038/srep18006. 10.1038/srep18006 PubMed 26648024
Related items:
Commonly requested with: