Skip to main content
Addgene

mGarnet2-RITA
(Plasmid #104314)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104314 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen/ life technologies
  • Backbone size w/o insert (bp) 5428
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mGarnet2-RITA
  • Insert Size (bp)
    1494
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site Unknown (unknown if destroyed)
  • 5′ sequencing primer GGC TAA CTA GAG AAC CCA CTG
  • 3′ sequencing primer GGC AAC TAG AAG GCA CAG TC
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mGarnet2-RITA was a gift from Ulrich Nienhaus (Addgene plasmid # 104314 ; http://n2t.net/addgene:104314 ; RRID:Addgene_104314)

  • For your References section:

    A far-red emitting fluorescent marker protein, mGarnet2, for microscopy and STED nanoscopy. Matela G, Gao P, Guigas G, Eckert AF, Nienhaus K, Nienhaus GU. Chem Commun (Camb). 2017 Jan 10;53(5):979-982. doi: 10.1039/c6cc09081h. 10.1039/c6cc09081h PubMed 28044150
Related items:
Commonly requested with: