pGEX2TK-YAP-WW1-RRK
(Plasmid #104306)

• Purpose: bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated
• Depositing Lab: Marius Sudol
• Publication: WW domains of YAP as a wild type and its RRK mutant that binds to PPx(phospho)Y peptides (unpublished)

Backbone

• Vector backbone: pGEX-2TK
• Backbone manufacturer: Pharmacia
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: YAP WW1 domain
• Species: H. sapiens (human)
• Mutation: WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGAAATAGAATCGATAAA)
• Entrez Gene: YAP1 (a.k.a. COB1, YAP, YAP-1, YAP2, YAP65, YKI)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: pGEX-5'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The human WW domain in this plasmid was obtained from cDNA described in the following reference:

Sudol M et al. J Biol Chem. 1995 Jun 16;270(24):14733-41. https://www.ncbi.nlm.nih.gov/pubmed/7782338

Please see the following references for additional information regarding the WW domain of human YAP:

1. Chen HI and Sudol M. Proc Natl Acad Sci U S A. 1995 Aug 15;92(17):7819-23. https://www.ncbi.nlm.nih.gov/pubmed/7644498

2. Toepert F et al., Angew Chem Int Ed Engl. 2001 Mar 2;40(5):897-900. https://www.ncbi.nlm.nih.gov/pubmed/11241639