pHH0103 IQGAP1 WW domain
(Plasmid
#104279)
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PurposeBacterial expression of WW domain from IQGAP1
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Depositing Labs
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104279 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepHH0103
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameIQGAP1 WW domain
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SpeciesH. sapiens (human)
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Insert Size (bp)183
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Mutationcodon optimized for expression in bacteria
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Entrez GeneIQGAP1 (a.k.a. HUMORFA01, SAR1, p195)
- Promoter tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site SfiI (unknown if destroyed)
- 3′ cloning site NotI (unknown if destroyed)
- 5′ sequencing primer pGEX-5' (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Amino acid sequence: Linker - ENLYFQGRRASV(gagctc) and WW domain - AVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQ
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHH0103 IQGAP1 WW domain was a gift from Sachdev Sidhu & Marius Sudol (Addgene plasmid # 104279 ; http://n2t.net/addgene:104279 ; RRID:Addgene_104279)
