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pHH0103 ITCH WW domain #4
(Plasmid #104219)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104219 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pHH0103
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    ITCH WW domain #4
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    183
  • Mutation
    codon optimized for expression in bacteria
  • Entrez Gene
    ITCH (a.k.a. ADMFD, AIF4, AIP4, NAPP1)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer pGEX-5'
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Amino acid sequence: Linker - ENLYFQGRRASV(gagctc) and WW domain - NEKPLPEGWEMRFTVDGIPYFVDHNRRTTTYIDPRTGKS

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pHH0103 ITCH WW domain #4 was a gift from Sachdev Sidhu & Marius Sudol (Addgene plasmid # 104219 ; http://n2t.net/addgene:104219 ; RRID:Addgene_104219)
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