GST-AR-400-449
(Plasmid
#104201)
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PurposeExpresses human AR-400-449 in pGEX-4T-1 bacterial expression vector as GST fusion protein
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Depositing Lab
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Publication
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104201 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepGEX-4T-1
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Backbone manufacturerPharmacia
- Total vector size (bp) 4969
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL-1 blue
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert namehuman androgen receptor aa 400-449
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SpeciesH. sapiens (human)
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Insert Size (bp)150
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Entrez GeneAR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XhoI/SalI (destroyed during cloning)
- 5′ sequencing primer don't have (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
GST-AR-400-449 was a gift from Elizabeth Wilson (Addgene plasmid # 104201 ; http://n2t.net/addgene:104201 ; RRID:Addgene_104201)
Map uploaded by the depositor.
