GST-AR-4-52
(Plasmid #104199)

• Purpose: Expresses human AR-4-52 in in pGEX-4T-1 as GST fusion protein
• Depositing Lab: Elizabeth Wilson
• Publication: Bai et al Mol Cell Biol. 2005 Feb;25(4):1238-57.

Backbone

• Vector backbone: pGEX-4T-1
• Backbone manufacturer: Pharmacia
• Total vector size (bp): 4969
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): XL-1 blue
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: human androgen receptor aa 4-52
• Species: H. sapiens (human)
• Insert Size (bp): 147
• GenBank ID: AAA51886.1
• Entrez Gene: AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamH1 (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: don't have
• 3′ sequencing primer: don't have

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry