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Addgene

GST-AR-1-36
(Plasmid #104192)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104192 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-5x-1
  • Backbone manufacturer
    Pharmacia
  • Total vector size (bp) 4900
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    XL-1 blue
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    human androgen receptor aa 1-36
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    108
  • GenBank ID
    AAA51886.1
  • Entrez Gene
    AR (a.k.a. AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM)
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer don't have
  • 3′ sequencing primer don't have
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GST-AR-1-36 was a gift from Elizabeth Wilson (Addgene plasmid # 104192 ; http://n2t.net/addgene:104192 ; RRID:Addgene_104192)

  • For your References section:

    FXXLF and WXXLF sequences mediate the NH2-terminal interaction with the ligand binding domain of the androgen receptor. He B, Kemppainen JA, Wilson EM. J Biol Chem. 2000 Jul 28;275(30):22986-94. 10.1074/jbc.M002807200 PubMed 10816582
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