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Addgene

delta Zfp521
(Plasmid #104190)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104190 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV Sport6
  • Backbone size w/o insert (bp) 4400
  • Total vector size (bp) 7610
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Zfp521
  • Alt name
    Evi3
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3210
  • Mutation
    deletion of final 6 Zn fingers
  • Entrez Gene
    Zfp521 (a.k.a. B930086A16Rik, Evi3, Znf521)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer SP6 5'GATTTAGGTGACACTATAG
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    IMAGE clone - nucleotide accession number BC021376

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

deletion of final six Zn fingers

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    delta Zfp521 was a gift from Kathryn Hentges (Addgene plasmid # 104190 ; http://n2t.net/addgene:104190 ; RRID:Addgene_104190)

  • For your References section:

    Zfp521 promotes B-cell viability and cyclin D1 gene expression in a B cell culture system. AlDallal S, Wolton K, Hentges KE. Leuk Res. 2016 Jul;46:10-7. doi: 10.1016/j.leukres.2016.03.013. Epub 2016 Apr 9. 10.1016/j.leukres.2016.03.013 PubMed 27107743
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