CIBN-GFP-Sec61B
(Plasmid #104177)

• Purpose: Expresses fusion of CIB1 (1-170) with GFP and Sec61B
• Depositing Lab: Bianxiao Cui
• Publication: Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8.

Backbone

• Vector backbone: pEGFP
• Backbone size w/o insert (bp): 4000
• Total vector size (bp): 5500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CIBN-GFP-Sec61B
• Alt name: CIB1 (Arabidopsis)
• Alt name: GFP
• Alt name: Sec61B (human)
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Entrez Gene: CIB1 (a.k.a. AT4G34530, T4L20.110, T4L20_110, cryptochrome-interacting basic-helix-loop-helix 1)
• Entrez Gene: SEC61B
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: MfeI (not destroyed)
• 5′ sequencing primer: CMV forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mutations K93A, R94A, K106A, K107A are mutations of putative nuclear localisation signals in CIB1.