pBHt2
(Plasmid #104175)

• Purpose: (Empty Backbone) Empty backbone for Agrobacterium tumefaciens-mediated transformation of Fusarium oxysporum
• Depositing Lab: Seogchan Kang
• Publication: Mullins et al Phytopathology. 2001 Feb;91(2):173-80. doi: 10.1094/PHYTO.2001.91.2.173.

Backbone

• Vector backbone: pCAMBIA1300
• Backbone manufacturer: CAMBIA, Canberra, Australia
• Backbone size (bp): 8960
• Modifications to backbone: pCAMBIA1300 was digested with with XhoI and BstXIA to remove the hph gene under control of the Cauliflower mosaic virus 35S (CaMV35S). The ends were blunted and ligated to a 1.4-kb HpaI fragment of pCB1004 containing the hph gene under the Aspergillus nidulans trpC promoter. The 3′ end of the hph gene was then ligated to the CaMV35S poly (A) termination signal.
• Vector type: Fungal expression
• Promoter: none
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: unknown

Resource Information

• A portion of this plasmid was derived from a plasmid made by: pCB1004: Carroll, A. M., Sweigard, J. A., and Valent, B. 1994. Improved vectors for selecting resistance to hygromycin. Fungal Genet. Newsl. 41:22.
• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry