pRG2 Citations (25)

Originally described in: Rescue of high-specificity Cas9 variants using sgRNAs with matched 5' nucleotides.
Kim S, Bae T, Hwang J, Kim JS Genome Biol. 2017 Nov 15;18(1):218. doi: 10.1186/s13059-017-1355-3. PubMed Journal

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Articles Citing pRG2

Articles
In vivo gene correction with targeted sequence substitution through microhomology-mediated end joining. Shin JH, Jung S, Ramakrishna S, Kim HH, Lee J. Biochem Biophys Res Commun. 2018 Jul 7;502(1):116-122. doi: 10.1016/j.bbrc.2018.05.130. Epub 2018 May 24. PubMed
Directed evolution of CRISPR-Cas9 to increase its specificity. Lee JK, Jeong E, Lee J, Jung M, Shin E, Kim YH, Lee K, Jung I, Kim D, Kim S, Kim JS. Nat Commun. 2018 Aug 6;9(1):3048. doi: 10.1038/s41467-018-05477-x. PubMed Associated Plasmids p3s-Sniper-Cas9
SpCas9 activity prediction by DeepSpCas9, a deep learning-based model with high generalization performance. Kim HK, Kim Y, Lee S, Min S, Bae JY, Choi JW, Park J, Jung D, Yoon S, Kim HH. Sci Adv. 2019 Nov 6;5(11):eaax9249. doi: 10.1126/sciadv.aax9249. eCollection 2019 Nov. PubMed
High-throughput analysis of the activities of xCas9, SpCas9-NG and SpCas9 at matched and mismatched target sequences in human cells. Kim HK, Lee S, Kim Y, Park J, Min S, Choi JW, Huang TP, Yoon S, Liu DR, Kim HH. Nat Biomed Eng. 2020 Jan;4(1):111-124. doi: 10.1038/s41551-019-0505-1. Epub 2020 Jan 14. PubMed
Purification of an Intact Human Protein Overexpressed from Its Endogenous Locus via Direct Genome Engineering. Yu J, Cho E, Choi YG, Jeong YK, Na Y, Kim JS, Cho SR, Woo JS, Bae S. ACS Synth Biol. 2020 Jul 17;9(7):1591-1598. doi: 10.1021/acssynbio.0c00090. Epub 2020 Jul 2. PubMed
Sequence-specific prediction of the efficiencies of adenine and cytosine base editors. Song M, Kim HK, Lee S, Kim Y, Seo SY, Park J, Choi JW, Jang H, Shin JH, Min S, Quan Z, Kim JH, Kang HC, Yoon S, Kim HH. Nat Biotechnol. 2020 Sep;38(9):1037-1043. doi: 10.1038/s41587-020-0573-5. Epub 2020 Jul 6. PubMed
Predicting the efficiency of prime editing guide RNAs in human cells. Kim HK, Yu G, Park J, Min S, Lee S, Yoon S, Kim HH. Nat Biotechnol. 2020 Sep 21. pii: 10.1038/s41587-020-0677-y. doi: 10.1038/s41587-020-0677-y. PubMed Associated Plasmids pLenti-PE2-BSD
Identification of Long Noncoding RNAs Involved in Differentiation and Survival of Vascular Smooth Muscle Cells. Lim YH, Ryu J, Kook H, Kim YK. Mol Ther Nucleic Acids. 2020 Aug 29;22:209-221. doi: 10.1016/j.omtn.2020.08.032. eCollection 2020 Dec 4. PubMed
Adenine base editing and prime editing of chemically derived hepatic progenitors rescue genetic liver disease. Kim Y, Hong SA, Yu J, Eom J, Jang K, Yoon S, Hong DH, Seo D, Lee SN, Woo JS, Jeong J, Bae S, Choi D. Cell Stem Cell. 2021 Sep 2;28(9):1614-1624.e5. doi: 10.1016/j.stem.2021.04.010. Epub 2021 May 4. PubMed
In vivo genome editing in single mammalian brain neurons through CRISPR-Cas9 and cytosine base editors. Song B, Kang CY, Han JH, Kano M, Konnerth A, Bae S. Comput Struct Biotechnol J. 2021 Apr 25;19:2477-2485. doi: 10.1016/j.csbj.2021.04.051. eCollection 2021. PubMed
In vivo gene editing via homology-independent targeted integration for adrenoleukodystrophy treatment. Hong SA, Seo JH, Wi S, Jung ES, Yu J, Hwang GH, Yu JH, Baek A, Park S, Bae S, Cho SR. Mol Ther. 2022 Jan 5;30(1):119-129. doi: 10.1016/j.ymthe.2021.05.022. Epub 2021 May 29. PubMed
Targeted mutagenesis in mouse cells and embryos using an enhanced prime editor. Park SJ, Jeong TY, Shin SK, Yoon DE, Lim SY, Kim SP, Choi J, Lee H, Hong JI, Ahn J, Seong JK, Kim K. Genome Biol. 2021 Jun 3;22(1):170. doi: 10.1186/s13059-021-02389-w. PubMed Associated Plasmids pCMV-CMP-PE-V1
Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases. Jang H, Jo DH, Cho CS, Shin JH, Seo JH, Yu G, Gopalappa R, Kim D, Cho SR, Kim JH, Kim HH. Nat Biomed Eng. 2021 Aug 26. pii: 10.1038/s41551-021-00788-9. doi: 10.1038/s41551-021-00788-9. PubMed Associated Plasmids TS-PE2-N TS-PE2-C-WPRE U6-pegRNA-H1-nick sgRNA-mCherry pLenti-NG-PE2-BSD
High-purity production and precise editing of DNA base editing ribonucleoproteins. Jang HK, Jo DH, Lee SN, Cho CS, Jeong YK, Jung Y, Yu J, Kim JH, Woo JS, Bae S. Sci Adv. 2021 Aug 27;7(35). pii: 7/35/eabg2661. doi: 10.1126/sciadv.abg2661. Print 2021 Aug. PubMed
High expression of uracil DNA glycosylase determines C to T substitution in human pluripotent stem cells. Park JC, Jang HK, Kim J, Han JH, Jung Y, Kim K, Bae S, Cha HJ. Mol Ther Nucleic Acids. 2021 Nov 29;27:175-183. doi: 10.1016/j.omtn.2021.11.023. eCollection 2022 Mar 8. PubMed
Cancer-cell-secreted miR-122 suppresses O-GlcNAcylation to promote skeletal muscle proteolysis. Yan W, Cao M, Ruan X, Jiang L, Lee S, Lemanek A, Ghassemian M, Pizzo DP, Wan Y, Qiao Y, Chin AR, Duggan E, Wang D, Nolan JP, Esko JD, Schenk S, Wang SE. Nat Cell Biol. 2022 May;24(5):793-804. doi: 10.1038/s41556-022-00893-0. Epub 2022 Apr 25. PubMed
Transient expression of an adenine base editor corrects the Hutchinson-Gilford progeria syndrome mutation and improves the skin phenotype in mice. Whisenant D, Lim K, Revechon G, Yao H, Bergo MO, Machtel P, Kim JS, Eriksson M. Nat Commun. 2022 Jun 2;13(1):3068. doi: 10.1038/s41467-022-30800-y. PubMed
Therapeutic base editing and prime editing of COL7A1 mutations in recessive dystrophic epidermolysis bullosa. Hong SA, Kim SE, Lee AY, Hwang GH, Kim JH, Iwata H, Kim SC, Bae S, Lee SE. Mol Ther. 2022 Aug 3;30(8):2664-2679. doi: 10.1016/j.ymthe.2022.06.005. Epub 2022 Jun 10. PubMed
TAPE-seq is a cell-based method for predicting genome-wide off-target effects of prime editor. Kwon J, Kim M, Bae S, Jo A, Kim Y, Lee JK. Nat Commun. 2022 Dec 29;13(1):7975. doi: 10.1038/s41467-022-35743-y. PubMed
Extru-seq: a method for predicting genome-wide Cas9 off-target sites with advantages of both cell-based and in vitro approaches. Kwon J, Kim M, Hwang W, Jo A, Hwang GH, Jung M, Kim UG, Cui G, Kim H, Eom JH, Hur JK, Lee J, Kim Y, Kim JS, Bae S, Lee JK. Genome Biol. 2023 Jan 10;24(1):4. doi: 10.1186/s13059-022-02842-4. PubMed
Transition Substitution of Desired Bases in Human Pluripotent Stem Cells with Base Editors: A Step-by-Step Guide. Park JC, Kim KT, Jang HK, Cha HJ. Int J Stem Cells. 2023 May 30;16(2):234-243. doi: 10.15283/ijsc22171. Epub 2023 Feb 28. PubMed
Prime editing with genuine Cas9 nickases minimizes unwanted indels. Lee J, Lim K, Kim A, Mok YG, Chung E, Cho SI, Lee JM, Kim JS. Nat Commun. 2023 Mar 30;14(1):1786. doi: 10.1038/s41467-023-37507-8. PubMed
Deep learning models to predict the editing efficiencies and outcomes of diverse base editors. Kim N, Choi S, Kim S, Song M, Seo JH, Min S, Park J, Cho SR, Kim HH. Nat Biotechnol. 2024 Mar;42(3):484-497. doi: 10.1038/s41587-023-01792-x PubMed Associated Plasmids SpCas9-NG-ABE8e(V106W) SpCas9-NG-ABE8.17-m+V106W SpCas9-NG-YE1-BE4max SpCas9-NG-SsAPOBEC3B SpCas9-NG-CGBE1 More ...
CRISPR prime editing for unconstrained correction of oncogenic KRAS variants. Jang G, Kweon J, Kim Y. Commun Biol. 2023 Jun 30;6(1):681. doi: 10.1038/s42003-023-05052-1. PubMed Associated Plasmids pCMV-PEmax-SpG pCMV-PEmax-SpG-P2A-hMLH1dn
Precise base editing without unintended indels in human cells and mouse primary myoblasts. Yoon DE, Kim NR, Park SJ, Jeong TY, Eun B, Cho Y, Lim SY, Lee H, Seong JK, Kim K. Exp Mol Med. 2023 Dec;55(12):2586-2595. doi: 10.1038/s12276-023-01128-4. Epub 2023 Dec 1. PubMed

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