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Addgene

pTP434
(Plasmid #104167)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104167 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE80 derivative
  • Total vector size (bp) 4687
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti-GFP nanobody Enhancer PDB 3K1K (3x Cysteine)
  • Species
    Vicugna pacos
  • Mutation
    3x Cysteines located at bps 493-495, 520-522, and 895-897 in full sequence
  • Tags / Fusion Proteins
    • 14x Histidine tag (N terminal on backbone)
    • NEDD8 from Brachypodium distachyon (cleavable by NEDP1) (N terminal on backbone)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP434 was a gift from Dirk Görlich (Addgene plasmid # 104167 ; http://n2t.net/addgene:104167 ; RRID:Addgene_104167)

  • For your References section:

    Nanobodies: site-specific labeling for super-resolution imaging, rapid epitope-mapping and native protein complex isolation. Pleiner T, Bates M, Trakhanov S, Lee CT, Schliep JE, Chug H, Bohning M, Stark H, Urlaub H, Gorlich D. Elife. 2015 Dec 3;4:e11349. doi: 10.7554/eLife.11349. 10.7554/eLife.11349 PubMed 26633879
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