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PurposeBacterial expression plasmid of anti-mouse kappa chain nanobody TP1170 (1x Cysteine)
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Depositing Lab
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Sequence Information
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Sequences (1) — Accept Affinity Reagent Sequence Policy
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Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104162 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepQE80 derivative
- Total vector size (bp) 4673
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
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SpeciesVicugna pacos
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Tags
/ Fusion Proteins
- 14xHistidine tag (N terminal on backbone)
- NEDD8 from Brachypodium distachyon (cleavable by NEDP1) (N terminal on backbone)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTP1174 was a gift from Dirk Görlich (Addgene plasmid # 104162 ; http://n2t.net/addgene:104162 ; RRID:Addgene_104162) -
For your References section:
A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies. Pleiner T, Bates M, Gorlich D. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. 10.1083/jcb.201709115 PubMed 29263082
