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Addgene

pTP1174
(Plasmid #104162)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104162 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pQE80 derivative
  • Total vector size (bp) 4673
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
  • Species
    Vicugna pacos
  • Tags / Fusion Proteins
    • 14xHistidine tag (N terminal on backbone)
    • NEDD8 from Brachypodium distachyon (cleavable by NEDP1) (N terminal on backbone)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTP1174 was a gift from Dirk Görlich (Addgene plasmid # 104162 ; http://n2t.net/addgene:104162 ; RRID:Addgene_104162)

  • For your References section:

    A toolbox of anti-mouse and anti-rabbit IgG secondary nanobodies. Pleiner T, Bates M, Gorlich D. J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20. 10.1083/jcb.201709115 PubMed 29263082
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