pGL3-BASIC-ARHGAP29 PROMOTER
(Plasmid
#104155)
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Purposeluciferase reporter with ARHGAP29 promoter
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 104155 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepGL3 basic
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Vector typeMammalian Expression, Luciferase
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameARHGAP29 promoter
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SpeciesH. sapiens (human)
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Insert Size (bp)1500
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Entrez GeneARHGAP29 (a.k.a. PARG1)
- Promoter ARHGAP29
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Tag
/ Fusion Protein
- luciferase (C terminal on backbone)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site KpnI (unknown if destroyed)
- 3′ cloning site HindIII (unknown if destroyed)
- 5′ sequencing primer RVprimer3 (Common Sequencing Primers)
Resource Information
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGL3-BASIC-ARHGAP29 PROMOTER was a gift from Marius Sudol (Addgene plasmid # 104155 ; http://n2t.net/addgene:104155 ; RRID:Addgene_104155) -
For your References section:
YAP Regulates Actin Dynamics through ARHGAP29 and Promotes Metastasis. Qiao Y, Chen J, Lim YB, Finch-Edmondson ML, Seshachalam VP, Qin L, Jiang T, Low BC, Singh H, Lim CT, Sudol M. Cell Rep. 2017 May 23;19(8):1495-1502. doi: 10.1016/j.celrep.2017.04.075. 10.1016/j.celrep.2017.04.075 PubMed 28538170