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Addgene

pcDNA3.1-HA-ARHGAP29
(Plasmid #104154)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104154 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pcDNA3.1
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ARHGAP29
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    3786
  • Entrez Gene
    ARHGAP29 (a.k.a. PARG1)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-HA-ARHGAP29 was a gift from Marius Sudol (Addgene plasmid # 104154 ; http://n2t.net/addgene:104154 ; RRID:Addgene_104154)

  • For your References section:

    YAP Regulates Actin Dynamics through ARHGAP29 and Promotes Metastasis. Qiao Y, Chen J, Lim YB, Finch-Edmondson ML, Seshachalam VP, Qin L, Jiang T, Low BC, Singh H, Lim CT, Sudol M. Cell Rep. 2017 May 23;19(8):1495-1502. doi: 10.1016/j.celrep.2017.04.075. 10.1016/j.celrep.2017.04.075 PubMed 28538170
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