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pNGVL4a-hCRTmE6mE7mL2
(Plasmid #104153)

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Item Catalog # Description Quantity Price (USD)
Plasmid 104153 Standard format: Plasmid sent in bacteria as agar stab 1 $85 *
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Backbone

  • Vector backbone
    pNGVL4a
  • Total vector size (bp) 7049
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    hCRTmE6mE7mL2 11-210
  • Alt name
    hCRT
  • Alt name
    MmuPV1 E6, MmuPV1 E7, MmuPV1 L2
  • Species
    H. sapiens (human); MmuPV1
  • Insert Size (bp)
    2624
  • Mutation
    residues 11 to 200 of the late protein L2
  • GenBank ID
    NP_004334.1
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Entrez Gene
    E6 (a.k.a. MmPv1_gp1)
  • Entrez Gene
    E7 (a.k.a. MmPv1_gp2)
  • Entrez Gene
    L2 (a.k.a. MmPv1_gp6)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (unknown if destroyed)
  • 3′ cloning site NotI (unknown if destroyed)
  • 5′ sequencing primer CGCAAATGGGCGGTAGGCGTG
  • 3′ sequencing primer GGGAAACGCCTGGTATCTTT
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pNGVL4a-hCRTmE6mE7mL2 was a gift from Richard Roden (Addgene plasmid # 104153 ; http://n2t.net/addgene:104153 ; RRID:Addgene_104153)

  • For your References section:

    Spontaneous and Vaccine-Induced Clearance of Mus Musculus Papillomavirus 1 Infection. Jiang RT, Wang JW, Peng S, Huang TC, Wang C, Cannella F, Chang YN, Viscidi RP, Best SRA, Hung CF, Roden RBS. J Virol. 2017 Jul 12;91(15). pii: e00699-17. doi: 10.1128/JVI.00699-17. Print 2017 Aug 1. 10.1128/JVI.00699-17 PubMed 28515303
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