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Addgene

pBAF
(Plasmid #104152)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104152 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET15B
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Propagate plasmid in strain such as DH5alpha. Use BL21 for expression. Do not allow cultures to reach stationary phase as this may result in loss of plasmid
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Barrier-to-autointegration factor (BAF)
  • Alt name
    BANF1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    267
  • GenBank ID
    AF070447.1
  • Entrez Gene
    BANF1 (a.k.a. BAF, BCRP1, D14S1460, NGPS)
  • Tag / Fusion Protein
    • N-terminal His tag with thrombin cleavage site (N terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBAF was a gift from Robert Craigie (Addgene plasmid # 104152 ; http://n2t.net/addgene:104152 ; RRID:Addgene_104152)

  • For your References section:

    A previously unidentified host protein protects retroviral DNA from autointegration. Lee MS, Craigie R. Proc Natl Acad Sci U S A. 1998 Feb 17;95(4):1528-33. PubMed 9465049
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