PAJ408
(Plasmid
#104150)
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Purpose(Empty Backbone) dCas9 plasmid expressing full length mKate2 mRNA without RBS
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104150 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonep15A
- Backbone size (bp) 9550
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Vector typeBacterial Expression, CRISPR
- Promoter pLtetO-1
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Cloning Information
- Cloning method Gibson Cloning
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
PAJ408 was a gift from Alfonso Jaramillo (Addgene plasmid # 104150 ; http://n2t.net/addgene:104150 ; RRID:Addgene_104150) -
For your References section:
Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, Jaramillo A. CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029. 10.1089/crispr.2020.0029 PubMed 33095053
Map uploaded by the depositor.
