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Addgene

PAJ390
(Plasmid #104148)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104148 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    p15A
  • Backbone size (bp) 9550
  • Vector type
    Bacterial Expression, CRISPR
  • Promoter pLtetO-1

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PAJ390 was a gift from Alfonso Jaramillo (Addgene plasmid # 104148 ; http://n2t.net/addgene:104148 ; RRID:Addgene_104148)
  • For your References section:

    Engineered RNA-Interacting CRISPR Guide RNAs for Genetic Sensing and Diagnostics. Galizi R, Duncan JN, Rostain W, Quinn CM, Storch M, Kushwaha M, Jaramillo A. CRISPR J. 2020 Oct;3(5):398-408. doi: 10.1089/crispr.2020.0029. 10.1089/crispr.2020.0029 PubMed 33095053