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pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA
(Plasmid #104140)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104140 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pSF4
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Renilla luciferase
  • Species
    Renilla reniformis
  • Insert Size (bp)
    936
  • Tags / Fusion Proteins
    • TOP element in 5'UTR
    • 24xPP7 stem loops in 3'UTR
    • xrRNA1 and 2 pseudo knots in 3'UTR
    • 24xMS2 stem loops in 3'UTR

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSF4 TetCMV 5'TOP intron Renilla-STOP 24xPP7 1xfirefly-siRNA xrRNA12 24xMS2 SV40 CTE polyA was a gift from Jeffrey Chao (Addgene plasmid # 104140 ; http://n2t.net/addgene:104140 ; RRID:Addgene_104140)

  • For your References section:

    The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells. Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA. Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19. 10.1016/j.molcel.2017.09.030 PubMed 29056324
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