pDG02583
(Plasmid #104129)

• Purpose: Optimized bacterial expression plasmid of bdNEDP1 protease (Frey and Goerlich, 2014)
• Depositing Lab: Dirk Görlich
• Publication: Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154. doi: 10.1083/jcb.201709115. Epub 2017 Dec 20.

Backbone

• Vector backbone: pQE80 derivative
• Total vector size (bp): 6134
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: NEDD8-specific protease 1 (NEDP1)
• Alt name: SENP8
• Species: Brachypodium distachyon
• Tags / Fusion Proteins:
  • 14x Histidine tag (N terminal on backbone)
  • Maltose binding protein (N terminal on backbone)
  • Small ubiquitin-like modifier (SUMO) cleavage site from Brachypodium distachyon (N terminal on backbone)

Resource Information

• Articles Citing this Plasmid:
  • 5 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Supporting reference: A new set of highly efficient, tag-cleaving proteases for purifying recombinant proteins. J Chromatogr A. 2014 Apr 11;1337:95-105. doi: 10.1016/j.chroma.2014.02.029