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LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine
(Plasmid #104122)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104122 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pAAV-CAG-FLEX
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 10096
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    spine-jRGECO1a-P2A-QuasAr-dark Citrine
  • Species
    Synthetic
  • Insert Size (bp)
    4000
  • Promoter CAG

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer gcaacgtgctggttattgtg
  • 3′ sequencing primer ggcggacttgaagaagtcgt
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    LZF1604V2: CAG-spine-jRGECO1a-P2A-QuasAr3-dark Citrine was a gift from Adam Cohen (Addgene plasmid # 104122 ; http://n2t.net/addgene:104122 ; RRID:Addgene_104122)

  • For your References section:

    All-optical synaptic electrophysiology probes mechanism of ketamine-induced disinhibition. Fan LZ, Nehme R, Adam Y, Jung ES, Wu H, Eggan K, Arnold DB, Cohen AE. Nat Methods. 2018 Oct;15(10):823-831. doi: 10.1038/s41592-018-0142-8. Epub 2018 Oct 1. 10.1038/s41592-018-0142-8 PubMed 30275587
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