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Addgene

pAAV-Syn1-tTA
(Plasmid #104109)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104109 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    AAV2
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    tTA2
  • Species
    E. coli
  • Insert Size (bp)
    747
  • Promoter hSyn1

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer M13-Reverse
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The tTA2 gene derived from pTet-Off Advanced vector (Clontech).
  • Article Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAAV-Syn1-tTA was a gift from Takeshi Imai (Addgene plasmid # 104109 ; http://n2t.net/addgene:104109 ; RRID:Addgene_104109)

  • For your References section:

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags. Sakaguchi R, Leiwe MN, Imai T. Elife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350. 10.7554/eLife.40350 PubMed 30454553
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