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pBS-TRE-HaloTag-WPRE
(Plasmid #104108)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104108 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pBluescript SK(+)
  • Backbone manufacturer
    Stratagene
  • Modifications to backbone
    An Xho-Xho fragment containing TRE-SV40 poly A was transferred from pTRE-Tight (Clontech).
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HaloTag
  • Species
    Rhodococcus rhodochrous
  • Insert Size (bp)
    894
  • Promoter TRE

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer CMV-F
    • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The HaloTag gene was from Promega.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBS-TRE-HaloTag-WPRE was a gift from Takeshi Imai (Addgene plasmid # 104108 ; http://n2t.net/addgene:104108 ; RRID:Addgene_104108)

  • For your References section:

    Bright multicolor labeling of neuronal circuits with fluorescent proteins and chemical tags. Sakaguchi R, Leiwe MN, Imai T. Elife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350. 10.7554/eLife.40350 PubMed 30454553
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