pCAG-tTA
(Plasmid #104102)

• Purpose: Expresses tTA2 under the CAG promoter in mammalian cells.
• Depositing Lab: Takeshi Imai
• Publication: Sakaguchi et al Elife. 2018 Nov 20;7. pii: 40350. doi: 10.7554/eLife.40350.

Backbone

• Vector backbone: pCAGEN
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tTA2
• Species: E. coli
• Insert Size (bp): 747
• Promoter: CAG

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: pCAG-F

Resource Information

• Supplemental Documents:
  • pCAG-tTA2.ape
• A portion of this plasmid was derived from a plasmid made by: pTet-Off Advanced from Clontech
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry