-
PurposeLentiviral vector expressing synonymous tandem PP7 coat protein fused to synonymous tandem GFP. Used to label reporter mRNAs.
-
Depositing Lab
-
Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 104099 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
-
Vector backbonepHAGE-UbiC
-
Vector typeMammalian Expression, Lentiviral
Growth in Bacteria
-
Bacterial Resistance(s)Ampicillin, 100 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)NEB Stable
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert namestdPCP-stdGFP
-
SpeciesSynthetic
-
Insert Size (bp)2295
-
Tags
/ Fusion Proteins
- N-terminal NLS
- Two synonymous copies of the PP7 coat protein
- Two synonymous copies of GFP (C-terminal)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GTGTGGCACAGCTAGTTCC (Common Sequencing Primers)
Resource Information
-
Articles Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
( Back to top)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
phage UbiC NLS HA stdPCP stdGFP was a gift from Jeffrey Chao (Addgene plasmid # 104099 ; http://n2t.net/addgene:104099 ; RRID:Addgene_104099) -
For your References section:
The Dynamics of mRNA Turnover Revealed by Single-Molecule Imaging in Single Cells. Horvathova I, Voigt F, Kotrys AV, Zhan Y, Artus-Revel CG, Eglinger J, Stadler MB, Giorgetti L, Chao JA. Mol Cell. 2017 Nov 2;68(3):615-625.e9. doi: 10.1016/j.molcel.2017.09.030. Epub 2017 Oct 19. 10.1016/j.molcel.2017.09.030 PubMed 29056324
