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Bassik Mouse CRISPR Knockout Libraries
(Pooled Libraries #1000000121, #1000000122, #1000000123, #1000000124, #1000000125, #1000000126, #1000000127, #1000000128, #1000000129, #1000000130)

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  • Purpose

    This lentiviral genome-scale CRISPR library targets all ~23,000 protein-coding genes in the mouse genome. The library is divided into 10 sublibraries defined by biological categories, and each of these is then split randomly by gene into two half libraries (A and B).

    This library includes safe-targeting controls, gRNAs which target the genome but are not expected to disrupt gene function, in addition to nontargeting controls. The gRNAs vary in length from 17–20 bp, with the truncated gRNAs (17 or 18 bp) having less off-target activity.

  • Vector Backbone

    pMCB320 (Plasmid #89359) - does not express Cas9

Ordering

Item Catalog # Description Quantity Price (USD)
Pooled Library 1000000121 Mouse CRISPR Deletion Library - Apoptosis and cancer† 1 $540 Add to Cart
Pooled Library 1000000122 Mouse CRISPR Deletion Library - Drug targets, kinases, phosphatases† 1 $540 Add to Cart
Pooled Library 1000000123 Mouse CRISPR Deletion Library - Gene expression† 1 $540 Add to Cart
Pooled Library 1000000124 Mouse CRISPR Deletion Library - Membrane proteins† 1 $540 Add to Cart
Pooled Library 1000000125 Mouse CRISPR Deletion Library - Stress proteostasis† 1 $540 Add to Cart
Pooled Library 1000000126 Mouse CRISPR Deletion Library - Trafficking, mitochondrial, motility† 1 $540 Add to Cart
Pooled Library 1000000127 Mouse CRISPR Deletion Library - Unassigned 1† 1 $540 Add to Cart
Pooled Library 1000000128 Mouse CRISPR Deletion Library - Unassigned 2† 1 $540 Add to Cart
Pooled Library 1000000129 Mouse CRISPR Deletion Library - Mouse unique 1† 1 $540 Add to Cart
Pooled Library 1000000130 Mouse CRISPR Deletion Library - Mouse unique 2† 1 $540 Add to Cart
Available to Academic and Nonprofits Only

  • † A Cas9 plasmid is NOT included with this item and will have to be ordered separately. Can be used with plasmids expressing Cas9 such as lentiCas9-Blast (#52962) or otherwise used with cell lines already expressing Cas9.

  • If you would like to receive all ten sublibraries in a single order, please email [email protected] for pricing (if you use a distributor in your country, please contact your distributor directly for pricing).

Library Details

Pooled Library Genes Targeted+ gRNAs+
1000000121 ~1,500 ~16,000
1000000122 ~1,000 ~11,000
1000000123 ~960 ~10,000
1000000124 ~470 ~5,500
1000000125 ~1,400 ~15,000
1000000126 ~970 ~10,000
1000000127 ~1,000 ~10,500
1000000128 ~1,050 ~11,000
1000000129 ~1,460 ~15,000
1000000130 ~1,580 ~16,500

  • + For each half sublibrary (A and B)

  • Controls
    7,500 unique non-targeting (375 per sublibrary); 7,500 unique safe-targeting (375 per sublibrary)
  • Species
    Mouse
  • Lentiviral Generation
    3rd

Library Shipping

This library is delivered as suspended DNA in two microcentrifuge tubes on blue ice. Each tube contains one half sublibrary. The tubes' contents will not necessarily be frozen. For best results, minimize freeze/thaws.

  • Volume
    ∼10 µL
  • Concentration
    10 ng/µL

Resource Information

Depositor Comments

The bioinformatic pipeline for analysis is available at bitbucket.org/dmorgens/castle and is described in Morgens et al. Nature Biotechnology 2016.


Figure 1. Sublibrary composition of mouse libraries

How to cite this pooled library ( Back to top )

These pooled libraries were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Mouse CRISPR Deletion Library - Apoptosis and cancer was a gift from Michael Bassik (Addgene #1000000121)
    Mouse CRISPR Deletion Library - Drug targets, kinases, phosphatases was a gift from Michael Bassik (Addgene #1000000122)
    Mouse CRISPR Deletion Library - Gene expression was a gift from Michael Bassik (Addgene #1000000123)
    Mouse CRISPR Deletion Library - Membrane proteins was a gift from Michael Bassik (Addgene #1000000124)
    Mouse CRISPR Deletion Library - Stress proteostasis was a gift from Michael Bassik (Addgene #1000000125)
    Mouse CRISPR Deletion Library - Trafficking, mitochondrial, motility was a gift from Michael Bassik (Addgene #1000000126)
    Mouse CRISPR Deletion Library - Unassigned 1 was a gift from Michael Bassik (Addgene #1000000127)
    Mouse CRISPR Deletion Library - Unassigned 2 was a gift from Michael Bassik (Addgene #1000000128)
    Mouse CRISPR Deletion Library - Mouse unique 1 was a gift from Michael Bassik (Addgene #1000000129)
    Mouse CRISPR Deletion Library - Mouse unique 2 was a gift from Michael Bassik (Addgene #1000000130)

  • For your References section:

    Genome-scale measurement of off-target activity using Cas9 toxicity in high-throughput screens. Morgens DW, Wainberg M, Boyle EA, Ursu O, Araya CL, Tsui CK, Haney MS, Hess GT, Han K, Jeng EE, Li A, Snyder MP, Greenleaf WJ, Kundaje A, Bassik MC. Nat Commun. 2017 May 5;8:15178. doi: 10.1038/ncomms15178. PubMed 28474669
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