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Cherry-HA-KCC2
(Plasmid #104077)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104077 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherry-C1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4731
  • Modifications to backbone
    CMV promoter replaced by UbC promoter
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    KCC2 (b isoform)
  • Alt name
    Slc12a5
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    3350
  • Mutation
    EcoR1 site in rat KCC2 was removed by silent mutation, A3267G in rat coding sequence
  • Entrez Gene
    Slc12a5 (a.k.a. Kcc2)
  • Promoter UbC
  • Tag / Fusion Protein
    • mCherry-HA tag (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site Eco R1 (unknown if destroyed)
  • 5′ sequencing primer unknown
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Cherry-HA-KCC2 was a gift from Igor Medyna (Addgene plasmid # 104077 ; http://n2t.net/addgene:104077 ; RRID:Addgene_104077)

  • For your References section:

    Genetically encoded impairment of neuronal KCC2 cotransporter function in human idiopathic generalized epilepsy. Kahle KT, Merner ND, Friedel P, Silayeva L, Liang B, Khanna A, Shang Y, Lachance-Touchette P, Bourassa C, Levert A, Dion PA, Walcott B, Spiegelman D, Dionne-Laporte A, Hodgkinson A, Awadalla P, Nikbakht H, Majewski J, Cossette P, Deeb TZ, Moss SJ, Medina I, Rouleau GA. EMBO Rep. 2014 Jul;15(7):766-74. doi: 10.15252/embr.201438840. Epub 2014 Jun 13. 10.15252/embr.201438840 PubMed 24928908
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