pTR47m4
(Plasmid #104068)

• Purpose: Luciferase Reporter for Formaldehyde Sensing
• Depositing Lab: David Liu
• Publication: Woolston et al Biotechnol Bioeng. 2017 Sep 16. doi: 10.1002/bit.26455.

Backbone

• Vector backbone: SC101
• Backbone manufacturer: David Liu Lab
• Backbone size w/o insert (bp): 2772
• Total vector size (bp): 5736
• Modifications to backbone: None known, possible mutations accumulated through subcloning
• Vector type: Bacterial Expression, Luciferase, Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Carbenicillin should be used at 50ug/mL to prevent selection for higher copy mutants of SC101 origin during growth
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: xluxAB
• Alt name: Luciferase
• Species: Photorabdus luminescens
• Insert Size (bp): 2124

Gene/Insert 2

• Gene/Insert name: frmR
• Alt name: Formaldehyde Repressor Protein
• Species: E. coli
• Insert Size (bp): 276
• GenBank ID: AP009048.1

Gene/Insert 3

• Gene/Insert name: frm Promoter m4
• Alt name: pfrm m4
• Species: E. coli
• Insert Size (bp): 36
• Mutation: 23r and 25th nucleotides in sequence mutated from C to A and T, respectively

Resource Information

• Supplemental Documents:
  • pTR47m4 - AP SC101 tetR-frmR frmOPm4-SD8_luxAB.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry