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Addgene

CRY2low-tdTomato
(Plasmid #104067)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 104067 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pmCherryN1
  • Backbone size w/o insert (bp) 4000
  • Total vector size (bp) 6900
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CRY2low-tdTomato
  • Alt name
    CRY2PHR
  • Alt name
    tdTomato
  • Species
    A. thaliana (mustard weed)
  • Mutation
    CRY2 aa 1-491 truncation, R489E A491D
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XmaI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV forward
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRY2low-tdTomato was a gift from Bianxiao Cui (Addgene plasmid # 104067 ; http://n2t.net/addgene:104067 ; RRID:Addgene_104067)

  • For your References section:

    Understanding CRY2 interactions for optical control of intracellular signaling. Duan L, Hope J, Ong Q, Lou HY, Kim N, McCarthy C, Acero V, Lin MZ, Cui B. Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8. 10.1038/s41467-017-00648-8 [pii] PubMed 28916751
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