CRY2low-mCherry-Raf1
(Plasmid #104066)

• Purpose: Expresses fusion of CRY2low mutant (CRY2 aa1-491 R489E A491D) with mCherry and Raf1
• Depositing Lab: Bianxiao Cui
• Publication: Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 10.1038/s41467-017-00648-8.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5300
• Total vector size (bp): 9500
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: CRY2low-mCherry-Raf1
• Alt name: CRY2PHR
• Alt name: mCherry
• Alt name: Raf1
• Species: H. sapiens (human), A. thaliana (mustard weed)
• Mutation: CRY2 aa 1-491 truncation, R489E A491D
• Entrez Gene: CRY2 (a.k.a. AT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2)
• Entrez Gene: RAF1 (a.k.a. CMD1NN, CRAF, NS5, Raf-1, c-Raf)
• Promoter: CMV

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: CMV forward

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry