pAAV-MBP-2xNLS-tdTomato
(Plasmid #104054)

• Purpose: An AAV genome encoding expression of the nuclear localized fluorescent protein tdTomato from the MBP promoter
• Depositing Lab: Viviana Gradinaru
• Publication: Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi: 10.1038/s41596-018-0097-3.

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Backbone

• Vector backbone: pAAV
• Backbone size w/o insert (bp): 6023
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: 2xNLS-tdTomato
• Insert Size (bp): 1492
• Promoter: MBP
• Tags / Fusion Proteins:
  • NLS (N terminal on insert)
  • NLS (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI/Acc56I (not destroyed)
• 3′ cloning site: EcoRI/EcoRV (not destroyed)

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://www.biorxiv.org/content/early/2018/01/19/246405 for bioRxiv preprint.