pEB1-mVenus ME
(Plasmid
#103989)
-
PurposePlasmid encoding mVenus ME
-
Depositing Lab
-
Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
---|---|---|---|---|---|
Plasmid | 103989 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
-
Vector backbonepEB1
- Backbone size w/o insert (bp) 3674
- Total vector size (bp) 4391
-
Modifications to backboneDerived from pUA66. Inserted constitutive promoter proC. Replaced original insert with FP of interest.
-
Vector typeBacterial Expression ; Low copy
Growth in Bacteria
-
Bacterial Resistance(s)Kanamycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)MG1655
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert namemVenus ME
-
SpeciesSynthetic
-
Insert Size (bp)717
-
MutationMutations relative to wild-type GFP (F46L, F64L, S65G, V68L, Q69M, S72A, M153T, V163A, S175G, T203Y, A206K)
- Promoter proC
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer GCGTATCACGAGGCCCTTTC
- 3′ sequencing primer AAAGGGAAAACTGTCCATATGCAC (Common Sequencing Primers)
Resource Information
-
Supplemental Documents
-
Article Citing this Plasmid
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
This FP derives from wtGFP. In this plasmid, the FP is coded with the original wtGFP nucleotide coding sequence except where mutations specific to the FP occur (see map). Please see "Notes On Plasmids In This Collection" document for further details and references.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pEB1-mVenus ME was a gift from Philippe Cluzel (Addgene plasmid # 103989 ; http://n2t.net/addgene:103989 ; RRID:Addgene_103989) -
For your References section:
Systematic characterization of maturation time of fluorescent proteins in living cells. Balleza E, Kim JM, Cluzel P. Nat Methods. 2018 Jan;15(1):47-51. doi: 10.1038/nmeth.4509. Epub 2017 Nov 20. 10.1038/nmeth.4509 PubMed 29320486