pcDNA3.1-R2ACR_142-EYFP
(Plasmid #103964)

• Purpose: Highly efficient anion channelrhodopsin from Rhodomonas sp. (CCAC1630) for expression in mammalian cells
• Depositing Lab: John Spudich
• Publication: Govorunova et al Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358.

Backbone

• Vector backbone: pcDNA3.1
• Backbone manufacturer: Invitrogen
• Backbone size w/o insert (bp): 6217
• Total vector size (bp): 7800
• Modifications to backbone: EYFP was cloned between NotI and XbaI sites
• Vector type: Mammalian Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Anion channelrhodopsin R2ACR_142
• Species: Rhodomonas sp.
• Insert Size (bp): 885
• Mutation: human codon optimized
• GenBank ID: KX879690
• Promoter: CMV
• Tag / Fusion Protein:
  • EYFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: T7

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry