AFDN_PDZ_1
(Plasmid #103938)

• Purpose: Protein expression and purification of TRITHORAX PDZ domain
• Depositing Lab: Sachdev Sidhu
• Publication: Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 10.1371/journal.pbio.0060239.

Backbone

• Vector backbone: pGEX-6P-1
• Backbone size w/o insert (bp): 4984
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: AFDN
• Alt name: TRITHORAX PDZ
• Species: H. sapiens (human)
• Mutation: contains AA983-1110 of AFDN
• Entrez Gene: AFDN (a.k.a. AF6, MLL-AF6, MLLT4, l-afadin)
• Promoter: tac
• Tag / Fusion Protein:
  • GST (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: unknown (unknown if destroyed)
• 3′ cloning site: unknown (unknown if destroyed)
• 5′ sequencing primer: pGEX Fwd

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry