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Addgene

HTRA1_PDZ_1
(Plasmid #103912)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 103912 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pGEX-6P-1
  • Backbone size w/o insert (bp) 4984
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    HTRA1
  • Alt name
    HTRA1 PDZ
  • Species
    H. sapiens (human)
  • Entrez Gene
    HTRA1 (a.k.a. ARMD7, CADASIL2, CARASIL, HtrA, L56, ORF480, PRSS11)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer pGEX Fwd
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS results found E476A within the HTRA1 translation compared to NP_002766.1

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HTRA1_PDZ_1 was a gift from Sachdev Sidhu (Addgene plasmid # 103912 ; http://n2t.net/addgene:103912 ; RRID:Addgene_103912)

  • For your References section:

    A specificity map for the PDZ domain family. Tonikian R, Zhang Y, Sazinsky SL, Currell B, Yeh JH, Reva B, Held HA, Appleton BA, Evangelista M, Wu Y, Xin X, Chan AC, Seshagiri S, Lasky LA, Sander C, Boone C, Bader GD, Sidhu SS. PLoS Biol. 2008 Sep 30;6(9):e239. doi: 10.1371/journal.pbio.0060239. 10.1371/journal.pbio.0060239 PubMed 18828675
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