b2-TS3
(Plasmid #103877)

• Purpose: full-length human b2 with tension sensor at b2_733
• Depositing Lab: Timothy Springer
• Publication: Nordenfelt et al Nat Commun. 2016 Oct 10;7:13119. doi: 10.1038/ncomms13119.

Backbone

• Vector backbone: pcDNA3.1/hygro(-)
• Backbone size w/o insert (bp): 5400
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: full-length human b2 with tension sensor at b2_733
• Alt name: b2-TS3
• Alt name: beta2-TS3
• Alt name: integrin subunit beta 2
• Species: H. sapiens (human)
• Insert Size (bp): 4200
• Entrez Gene: ITGB2 (a.k.a. CD18, LAD, LCAMB, LFA-1, MAC-1, MF17, MFI7)
• Tag / Fusion Protein:
  • tension sensor module (C terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (unknown if destroyed)
• 3′ cloning site: HIndIII (unknown if destroyed)
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7

Resource Information

• Supplemental Documents:
  • b2-TS3 information

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid is identical to Plasmid 104353: pcDNA3.1/Hygro(-)-B2-TS3 (https://www.addgene.org/104353/)