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Addgene

pC0052-REPAIR non-targeting guide clone into pC0043 Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb DNA ladder and a digestion of plasmid 103868.

We predict the following pattern:

SapI: 3 kb
SacI-HF: 3 kb
SapI/SacI-HF: 2.4 kb and 0.6 kb

We see the expected result.