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Addgene

pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A Notes

Addgene Diagnostic Digest

This gel depicts the NEB 1kb Ladder and a digestion of the plasmid
pC0049-EF1a-dPSPCas13b-NES-HIV, H133A/H1058A with the
following enzyme(s): NotI-HF, NheI-HF, KpnI-HF, and SpeI-HF
Expected bands:
NotI-HF/NheI-HF: Two bands: 7376 bp and 5231 bp
KpnI-HF/SpeI-HF: Two bands: 7773 bp and 4834 bp
Results:
We observed the expected bands for this digest.