pC0047-CMV-dPspCas13b-ADAR1DD(E1008Q)
(Plasmid #103863)

• Purpose: dPspCas13b-ADAR1DD(E1008Q) fusion that can be used to selectively edit adenosine to inosine in RNA molecules when used in conjuction with a guide RNA
• Depositing Lab: Feng Zhang
• Publication: Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi: 10.1126/science.aaq0180.

Backbone

• Vector backbone: pcDNA3.1
• Backbone size w/o insert (bp): 5428
• Total vector size (bp): 9705
• Vector type: Mammalian Expression, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: PspCas13b
• Species: Prevotella sp. P5-125
• Insert Size (bp): 3270
• Mutation: Changed histidne 133 to alanine and histidine 1058 to alanine to remove RNAse activity.
• GenBank ID: WP_044065294

Gene/Insert 2

• Gene/Insert name: huADAR1DD
• Alt name: human ADAR1 deaminase domain
• Species: H. sapiens (human)
• Insert Size (bp): 1011
• Mutation: Only the deaminase domain (AA 886-1226 are expressed). Glutamate 1008 is mutated to glutamine.
• Entrez Gene: ADAR (a.k.a. ADAR1, AGS6, DRADA, DSH, DSRAD, G1P1, IFI-4, IFI4, K88DSRBP, P136)

Resource Information

• Supplemental Documents:
  • pc0047-cmv-dpspcas13b-adar1dd-e1008q.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Benchling link: https://benchling.com/s/seq-R3zRpb4whgEiZBoTvpgM